You can check your Twilio account balance using Python by first setting up the necessary environment variables with your account SID and Auth Token. Then, you can use the Twilio Python Helper library to initialize a client instance and fetch your account balance. The balance is returned as a string, so it's converted to a float for easier usage. You can also retrieve the currency used in your account. Finally, you can integrate this into a simple script that prints out your current account balance when run from the command line.