In the field of medical imaging and computer vision models, DICOM and NIfTI files are highly specialized and require specific practices for accurate results. Data security is crucial in this sector due to strict regulatory oversights worldwide, such as HIPAA and SOC 2. Healthcare providers primarily use DICOM and NIfTI imaging standards, which differ from PACS and JPEG formats. Four best practices for using these file formats in computer vision models include displaying data correctly for pixel-perfect annotations, ensuring high levels of medical image annotation quality, making data audits granular for regulatory compliance, and improving efficiency with automation features to save radiologists time.